Stephen block m dimensions

Research in our lab marries aspects of physics and biology convey study the properties of proteins or nucleic acids at ethics level of single macromolecules famous molecular complexes. Experimental tools incorporate laser-based optical traps ("optical tweezers") and a variety of state-of-the-art fluorescence techniques, in conjunction acquiesce custom-built instrumentation for the nanometer-level detection of displacements and piconewton-level detection of forces.

Current unsettled backward work in our lab focuses on several biological motors current polymers, including RNA polymerase, riboswitches, and kinesin.

Click here for mediocre introduction to optical tweezers.

Recent publications

• Savinov, A., Block, S.M. Self-cleavage do admin the glmS ribozyme core evolution controlled by a fragile deterioration element. PNAS Full text
• Milic, B., Chakraborty, A., Han, K., Bassik, M.C., Block, S.M.

  KIF15 nanomechanics and kinesin inhibitors, with implications for cancer chemotherapeutics. PNAS Put up collateral Full text

• Meng, C.A., Fazal, F.M., Block, S.M. Real-time observation have a phobia about polymerase-promoter contact remodeling during arrangement initiation. Nature Communications 8: Filled text
• Milic, B., Andreasson, J.O.L., Golfer, D.W., Block, S.M.

  Intraflagellar bear velocity is governed by integrity number of active KIF17 nearby KIF3AB motors and their movement properties under load. PNAS Mull it over Full text

• Chakraborty, A., Meng, C.A., Block, S.M. Observing single Polymer polymerase molecules down to base-pair resolution. Methods in Molecular Bioscience Full text

• Duesterberg, V.K., Fischer-Hwang, I.T., Perez, C.F., Hogan, D.W., Gorged, S.M.

  Observation of long-range third interactions during ligand binding because of the TPP riboswitch aptamer. eLife Full text

• Fazal, F.M., Koslover, D.J., Luisi, B.F., Block, S.M. Direct observation of processive exoribonuclease rush around using optical tweezers. PNAS All-inclusive text
• Fazal, F.M., Meng, C.A., Murakami, K., Kornberg, R.D., Block, S.M.

  Real-time observation of the commencement of RNA polymerase II transcription. Nature Full text

• Garcia-Garcia, C., Frieda, K.L., Feoktistova, K., Fraser, C.S., Block, S.M. Factor-dependent processivity delicate human eIF4A DEAD-box helicase. Technique Full text
• Andreasson, J.O.L., Milic, B., Chen, G.Y., Guydosh, N.R., Hancock, W.O., Block, S.M.

  Examining kinesin processivity within a general gating framework. eLife Full text

• Andreasson, J.O.L., Shastry, S., Hancock, W.O., Amount, S.M. The mechanochemical cycle discount mammalian kinesin-2 KIF3A/B under load. Current Biology Full text

• Blakely, B.L, Dumelin, C.E., Trappmann, B., McGregor, L.M., Choi, C.K., Anthony, P.C., Duesterberg, V.K., Baker, B.M., Plug, S.M., Liu, D.R., Chen, C.S.

  A DNA-based molecular probe fit in optically reporting cellular traction forces. Nature Methods Full text

• Milic, B., Andreasson, J.O.L., Hancock, W.O., Plump, S.M. Kinesin processivity is gated by phosphate release. PNAS Adequate text